标题：山萘酚靶向脂质体的制备与评价

链接：https://xueshu.baidu.com/usercenter/paper/show?paperid=7798a3dbd8f6faa67daba37ba632887f&site=xueshu_se

作者：刘祖浩，雷玉杰，宋浩颖，常聪

摘要：

We have developed a new type of PEG-immunoliposome carrying monoclonal antibodies or their fragments (F(ab′)2, Fab′) at the distal ends of the PEG chains (Type C). Distearoylphosphatidylethanolamine derivatives of PEG with car☐yl group (DSPE-PEG-COOH) or dipalmitoyl phosphatidylethanolamine derivatives of PEG with maleimidyl group (DPPE-PEG-Mal) at the PEG terminal were newly synthesized. Small unilamellar liposomes (90–130 nm in diameter) were prepared from distearoyl phosphatidylcholine and cholesterol (2:1, m/m) containing 6 mol% of DSPE-PEG-COOH or DPPE-PEG-Mal. To target to the vascular endothelial lung surface as a model accessible site, 34A antibody, which is highly specific to mouse pulmonary endothelial cells, was conjugated to PEG-liposome (34A-Type C). The degree of lung binding of 34A-Type C in BALB/c mice was significantly higher than that of the 34A-Type A which is an ordinary type immunoliposome (without PEG derivatives).

译文：

我们开发了一种新型的PEG免疫脂质体，在PEG链的远端携带单克隆*体或其片段（F（ab′）2，Fab′）（C型）。聚乙二醇二硬脂酰磷脂酰乙醇胺衍生物☐新合成了PEG末端带有马来酰亚胺基的烷基（DSPE-PEG-COOH）或二棕榈酰磷脂酰乙醇胺衍生物（DPPE-PEG-Mal）。由含有6mol%DSPE-PEG-COOH或DPPE-PEG-Mal的二硬脂酰磷脂酰胆碱和胆固醇（2:1，m/m）制备了小单层脂质体（直径90-130nm）。为了靶向血管内皮肺表面作为模型可及位点，将对小鼠肺内皮细胞高度特异性的34A*体与PEG脂质体（34A C型）结合。BALB/C小鼠中34A C型的肺结合程度明显高于普通型免疫脂质体（不含PEG衍生物）34A A型。

DOI：10.3969/j.issn.1008-987x.2021.03.09

西安齐岳生物提供相关产品：

DSPE-PEG-acid

DSPE-PEG4-acid

DSPE-PEG-Ald

DSPE-PEG-Amine，1069-79-0

DSPE-PEG-azide

DSPE-PEG5-azide

DSPE-PEG-Biotin

DSPE-PEG-Boronate

DSPE-PEG-CH2COOH

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