去甲斑蝥素DSPE-PEG-MAL纳米粒的制备

文献链接：https://xueshu.baidu.com/usercenter/paper/show?paperid=1u7y0gj0ry6b0880de3f0ac0s6129880&site=xueshu_se

文献作者：JS Cudnik，PC West，RK Fisher，Sam Mattern-Schain，M Best，SS Kirkpatrick，MB Freeman，OH Grandas，DJ Mountain

目的:制备去甲斑蝥素DSPE-PEG-MAL纳米粒,探讨纳米粒接上碳酸酐酶Ⅸ*体后的*肿瘤作用变化. 

方法:将碳酸酐酶Ⅸ*体孵化修饰去甲斑蝥素DSPE-PEG-MAL纳米粒,考察其载药率,包封率,粒径等指标;通过MTT法考察去甲斑蝥素及载药纳米粒对A549的细胞生存率,为细胞摄取实验剂量设置提供依据;取荷瘤裸鼠分别尾静脉注射去甲斑蝥素和载药纳米粒,并观察裸鼠的饮食,精神状态,每周用游标卡尺测量肿瘤的大小,绘制肿瘤生长曲线. 

结果:制备的碳酸酐酶Ⅸ*体修饰的去甲斑蝥素纳米粒呈圆球状,载药量为(5.26±0.03)%,包封率为(80.93±1.01)%,粒径和Zeta电位分别为146.5±48.9nm,-(14.79±0.67)mv;去甲斑蝥素及载药纳米粒对肿瘤细胞的抑制作用呈剂量和时间依赖性,载药纳米粒对肿瘤细胞的抑制作用明显优于游离药物,且经碳酸酐酶Ⅸ*体修饰后纳米粒对A549的抑制率加强. 结论:去甲斑蝥素纳米粒经碳酸酐酶Ⅸ*体修饰后能达到主动靶向的效果,同时能与去甲斑蝥素协同抑瘤,起到一举多得的目的.

译文：

Objective: To prepare DSPE-PEG-MAL nanoparticles of norcantharidin and investigate the changes in anti-tumor activity of the nanoparticles after attaching carbonic anhydrase IX antibody  

Method: Carbonic anhydrase IX antibody was incubated and modified with norcantharidin DSPE-PEG-MAL nanoparticles to investigate their drug loading rate, encapsulation efficiency, particle size, and other indicators; Using MTT assay to investigate the cell survival rate of A549 cells treated with norcantharidin and drug loaded nanoparticles, providing a basis for setting the experimental dose for cell uptake; Tumor bearing nude mice were injected with norcantharidin and drug loaded nanoparticles via tail vein, and their diet and mental state were observed. The size of the tumor was measured weekly using a vernier caliper, and the tumor growth curve was plotted  

Result: The prepared carbonic anhydrase IX antibody modified norcantharidin nanoparticles were spherical in shape, with a drug loading of (5.26 ± 0.03)% and an encapsulation efficiency of (80.93 ± 1.01)%. The particle size and Zeta potential were 146.5 ± 48.9nm and - (14.79 ± 0.67) mv, respectively. The inhibitory effects of norcantharidin and drug loaded nanoparticles on tumor cells were dose-dependent and time-dependent, and the inhibitory effect of drug loaded nanoparticles on tumor cells was significantly better than that of the free drug. Moreover, the inhibitory rate of nanoparticles on A549 was enhanced after modification with carbonic anhydrase IX antibody Conclusion: Norcantharidin nanoparticles can achieve active targeting after modification with carbonic anhydrase IX antibody, and can synergistically inhibit tumors with Norcantharidin, achieving a win-win effect

DOI：10.3969/j.issn.1008-987x.2021.03.09

西安齐岳生物提供相关产品：

DSPE-PEG-Mal

DSPE-PEG-NH2

DSPE-PEG-NHS

DSPE-PEG-OH

DSPE-PEG-propargyl

DSPE-PEG-Alkyne

DSPE-PEG-Rhodamine

DSPE-Rhodamine

DSPE-PEG-SH

DSPE-PEG-SPDP

DSPE-SPDP

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