基于DSPE-PEG-DOTA的压电纳米平台在脑胶质瘤中的应用探索

链接：https://d.wanfangdata.com.cn/periodical/sdykdxxb202306002

作者： 陈治光  张巍  何文

摘要：

目的：探讨基于偏氟乙烯(vinylidene fluoride,VDF)和三氟乙烯(trifluoroethylene,TrFE)的共聚物[poly(VDF-TrFE),P(VDF-TrFE)]构建的复合压电纳米粒子在脑胶质瘤细胞中*和成像的效能.方法 对P(VDF-TrFE)压电材料进行晶型重塑和亲水性改善,通过点击化学反应将精氨酰-甘氨酰-天冬氨酸合成肽(Arg-Gly-Asp,RGD)连接到二硬脂酰基磷脂酰乙醇胺-聚乙二醇-马来酰亚胺脂[1,2-distearoyl-sn-glycero-3-phosphoethanolamine-poly(ethylene glycol)-maleimide,DSPE-PEG-Mal]上;同时以DSPE-PEG-四氮杂环配体(DSPE-PEG-tetranitroheterocyclic ligand,DSPE-PEG-DOTA)为底液,加入一定量醋酸钆溶液,通过螯合获得 DSPE-PEG-DOTA-钆造影剂(DSPE-PEG-DOTA-Gd);按比例将重塑后的 P(VDF-TrFE)、DAPE-PEG-Mal-RGD 和DSPE-PEG-DOTA-Gd混合,最后获得复合压电纳米粒子P(VDF-TrFE)@DOTA-Gd@RGD,对其进行表征以及用于细胞层面实验研究.结果 P(VDF-TrFE)@DOTA-Gd@RGD复合压电纳米粒子具有*的稳定性,尤其是在达尔伯克氏必需基本培养基(Dulbecco's modified eagle medium,DMEM)中,其粒径随时间变化保持在 160 nm左右;经过晶型重塑后,P(VDF-TrFE)@DOTA-Gd@RGD的压电性能明显提高,表面电势约为 4.2 mV;该纳米粒子可作为磁共振成像(magnetic resonance imaging,MRI)显像剂,而且在超声激发下,P(VDF-TrFE)@DOTA-Gd@RGD复合压电纳米粒子能产生大量活性氧,抑制了胶质瘤细胞的增殖、侵袭和迁移能力,同时还促进其凋亡.结论 P(VDF-TrFE)@DOTA-Gd@RGD复合压电纳米粒子作为MRI显像剂可以用于脑胶质瘤的定位,且可以作为一种声敏剂用于脑胶质瘤细胞的声动力*.

译文：

Objective To explore the efficacy of composite piezoelectric nanoparticles constructed based on poly(vinylidene fluoride)-trifluoroethylene[P(VDF-TrFE)]in the treatment and imaging of glioma.Methods P(VDF-TrFE)piezoelectric materials were reshaped in crystal form and improved in hydrophilicity,and Arg-Gly-Asp(RGD)targeted peptides were connected to 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-poly(ethylene glycol)-maleimide(DSPE-PEG-Mal)by click chemistry reaction.In addition,DSPE-PEG-tetranitroheterocyclic ligand(DSPE-PEG-DOTA)was mixed with a certain amount of gadolinium acetate solution was added to obtain DSPE-PEG-DOTA-Gd through chelation.And then the P(VDF-TrFE),DAPE-PEG-Mal RGD,and DSPE-PEG-DOTA-Gd in proportion were mixed to obtain composite piezoelectric nanoparticles P(VDF-TrFE)@DOTA-Gd@RGD.The obtained nanoparticles were characterized and assesed.Results P(VDF-TrFE)@DOTA-Gd@RGD composite piezoelectric nanoparticles have excellent stability,especially in Dulbecco's modified eagle medium(DMEM)solutions where their particle size remained around 160 nm over time.After crystal reshaping,the piezoelectric performance of P(VDF-TrFE)@DOTA-Gd@RGD is significantly improved,with a surface potential of approximately 4.2 mV.The nanoparticles could be used as a magnetic resonance imaging(MRI)agent.Under ultrasound excitation,P(VDF-TrFE)@DOTA-Gd@RGD composite piezoelectric nanoparticles generated a large amount of reactive oxygen species,inhibited the proliferation,invasion,and migration ability of glioma cells,and promoted their apoptosis.Conclusion P(VDF-TrFE)@DOTA-Gd@RGD composite piezoelectric nanoparticles could be used as an MRI agent for the localization of brain gliomas and as a sound sensitive agent for sonodynamic therapy of glioma.

DOI： 10.3969/j.issn.1006-7795.2023.06.002

西安齐岳生物提供相关产品：

mPEG-Palmitic acid

mPEG-STA

DSPE-mPEG

DSPE-Biotin

16:0 Biotinyl Cap PE

16:0 Folate Cap PE

DSPE-Thiol，DSPE-SH

DSPE-COOH，DSPE-Succinic acid

DSPE-MAL，DSPE-Maleimide

DSPE-NHS

DSPE-Hyaluronate

DSPE-Dextran

DSPE-Chitosan

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