链接：https://www.sciencedirect.com/science/article/pii/S1549963420301921

作者：赵 章亭，马兴 元，张瑞 环，胡发彪，张 桐，刘玉萍，Myong Hun Han，方 有，杨 易，郑文云

摘要：

DNA疫苗是预防和*传染病的一个有吸引力的免疫平台，但现有的缺点限制了它在临床前和临床试验中的应用，例如免疫原性弱和半衰期短。本文，我们报道了一种新型脂质体-聚合物混合纳米颗粒（pSFV-MEG/LNPs），由可生物降解的核心（mPEG-PLGA）和亲水外壳（卵磷脂/PEG-DSPE-Mal 2000）组成，用于递送多表位自复制DNA疫苗（pSFV-MEG）。与PBS相比，具有最佳粒径（161.61±15.63nm）和高包封率（87.60±8.73％）的pSFV-MEG/LNPs可诱导强烈的体液免疫反应（3.22倍）和细胞免疫反应（1.60倍）。此外，pSFV-MEG/LNPs的体液和细胞免疫应答分别是pSFV-MEG的1.58倍和1.05倍。所有结果证实，LNPs是一种非常有前景的增强pSFV-MEG体液和细胞免疫应答的工具。此外，该合理的设计和递送平台可用于开发其他传染病的DNA疫苗。

译文：

DNA vaccine is an attractive immune platform for the prevention and treatment of infectious diseases, but existing disadvantages limit its use in preclinical and clinical assays, such as weak immunogenicity and short half-life. Here, we reported a novel liposome-polymer hybrid nanoparticles (pSFV-MEG/LNPs) consisting of a biodegradable core (mPEG-PLGA) and a hydrophilic shell (lecithin/PEG-DSPE-Mal 2000) for delivering a multi-epitope self-replication DNA vaccine (pSFV-MEG). The pSFV-MEG/LNPs with optimal particle size (161.61 ± 15.63 nm) and high encapsulation efficiency (87.60 ± 8.73%) induced a strong humoral (3.22-fold) and cellular immune responses (1.60-fold) compared to PBS. Besides, the humoral and cellular immune responses of pSFV-MEG/LNPs were 1.58- and 1.05-fold than that of pSFV-MEG. All results confirmed that LNPs was a very promising tool to enhance the humoral and cellular immune responses of pSFV-MEG. In addition, the rational design and delivery platform can be used for the development of DNA vaccines for other infectious diseases.

西安齐岳生物提供相关产品：

DSPE-PEG-CPP

DSPE-PEG-octreotide

DSPE-PEG-SP94

DSPE-PEG-CCK8

DSPE-PEG2000-GE11

DSPE-PEG2K-YIGSR

DSPE-PEG2K-RVG29

DSPE-PEG2K-MMPs

DSPE-PEG2000-NGR

DSPE-PEG-GRGDS

DSPE-PEG2K-R8

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