链接:https://www.sciencedirect.com/science/article/pii/S2405580821001618
作者:滨进 酒井美嘉 板仓翔子 真岛英二 小暮健太郎
节选:
NIH3T3细胞(小鼠成纤维细胞株)购自日本理化学研究所BRC细胞库(日本和光)。A375细胞(人黑色素瘤细胞株)和HUEhT-2细胞(永生化人脐静脉内皮细胞株)购自日本JCRB细胞库(日本大阪)。3-(N-琥珀酰亚胺基氧戊二酰)氨基丙基聚乙二醇-氨基甲酰二硬脂酰磷脂酰乙醇胺(DSPE-PEG-NHS)、N-(琥珀酰亚胺基氧戊二酰)-L-α-磷脂酰乙醇胺(二硬脂酰)(DSPE-NHS)和1,2-二硬脂酰-sn-甘油-3-磷酸胆碱(DSPC)购自日本油脂株式会社(日本东京)。胆固醇、磷酸双十六烷基酯(DCP) 和DXR 盐酸盐购自 Sigma-Aldrich 公司(美国密苏里州圣路易斯)。1,2-二油酰-sn-甘油-3-磷酸乙醇胺-N-(丽丝胺罗丹明B磺酰基) (Rh-PE) 购自 Avanti Polar Lipid 公司(美国阿拉巴马州阿拉巴斯特)。*人 CD31 *体(小鼠IgG1 κ)、*人 CD147 *体(小鼠 IgG1 κ)和小鼠 IgG1 κ 同型对照购自 eBioscience 公司(美国加利福尼亚州圣地亚哥)。四甲基罗丹明(TRITC) 标记的兔*鼠多克隆*体购自 Dako 公司(丹麦格洛斯特鲁普)。
译文:
NIH3T3 cells, a mouse fibroblast cell line, were obtained from RIKEN BRC Cell Bank (Wako, Japan). A375 cells, a human melanoma cell line, and HUEhT-2 cells, an immortalized human umbilical vein endothelial cell line were obtained from the JCRB Cell Bank (Osaka, Japan). 3-(N-succinimidyloxyglutaryl) aminopropyl, polyethyleneglycol-carbamyl distearoylphosphatidyl-ethanolamine (DSPE-PEG-NHS), N-(Succinimidyloxy-glutaryl)-L-α-phosphatidylethanolamine, Distearoyl (DSPE-NHS) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) were obtained from NOF Corporation (Tokyo, Japan). Cholesterol, dihexadecyl phosphate (DCP) and DXR hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO, USA). 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rh-PE) were purchased from Avanti Polar Lipid (Alabaster, AL, U.S.A.). Anti-human CD31 antibody (mouse IgG1 κ), anti-human CD147 antibody (mouse IgG1 κ) and mouse IgG1 κ isotype control were purchased from eBioscience (San Diego, CA, USA). Tetramethylrhodamine (TRITC)-labeled rabbit anti-mouse polyclonal antibody was obtained from Dako (Glostrup, Denmark).
西安齐岳生物提供相关产品:
DSPE-PEG-iRGD
DSPE-PEG-多肽(各种定制)
DSPE-PEG-RGD
DSPE-PEG-c(RGDfk)
DSPE-PEG-c(RGDfK)-FITC
DSPE-PEG-c(RGDfK)-Biotin
DSPE-PEG-c(RGDfc)
DSPE-PEG-GE11
DSPE-PEG-PTP
DSPE-PEG-T7(HAIYPRH)
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