文献：HER2-specific affibody-conjugated thermosensitive liposomes (Affisomes) for improved delivery of anticancer agents.

文献链接：https://xueshu.baidu.com/usercenter/paper/show?paperid=b7066b9e17eebe26a02523fad0cc64f8&site=xueshu_se

作者：A Puri，G Kramer-Marek，R Campbell-Massa，A Yavlovich，SC Tele，SB Lee，JD Clogston，AK Patri，R Blumenthal，J Capala

摘要：

The effects of this modification on physical characteristics and stability of the resulting nanoparticles denoted as Affisomes were investigated. Thermosensitive small unilamellar vesicle (SUV) liposomes of (80100 nm) a diameter consisting of dipalmitoyl phosphatidylcholine (DPPC, Tm 41°C) as the matrix lipid and a maleimide-conjugated pegylated phospholipid (DSPE-MaL-PEG2000) were prepared by probe sonication. Fluorescent probes were incorporated into liposomes for biophysical and/or biochemical analysis and/or triggered-release assays. Affibody was conjugated to these liposomes via its C-terminal cysteine by incubation in the presence of a reducing agent (e.g., tributylphosphine) for 1620 hours under an argon atmosphere. 

研究了这种修饰对所得纳米颗粒（称为仿射体）的物理特性和稳定性的影响。通过探针超声处理制备了直径为（80100 nm）的热敏小单层囊泡（SUV）脂质体，由二棕榈酰磷脂酰胆碱（DPPC，Tm 41°C）作为基质脂质和马来酰亚胺偶联的聚乙二醇化磷脂（DSPE-MaL-PEG2000）组成。荧光探针被掺入脂质体中，用于生物物理和/或生化分析和/或触发释放分析。通过在还原剂（如三丁基膦）存在下在氩气气氛下孵育1620小时，将附着体通过其C-末端半胱氨酸与这些脂质体结合。

相关推荐：

DSPE-PEG-SC

DSPE-PEG-SCM

DSPE-PEG-SG

DSPE-PEG-SH

DSPE-PEG-Silane

DSPE-PEG-SP2-AA

DSPE-PEG-SS

DSPE-PEG-TCO

DSPE-PEG-Tetrazine

DSPE-PEG-TMS

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