DSPE-PEG-PLGA纳米系统增强姜黄素调控NCOA5表达-UDP糖丨MOF丨金属有机框架丨聚集诱导发光丨荧光标记推荐西安齐岳生物

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DSPE-PEG-PLGA纳米系统增强姜黄素调控NCOA5表达

发布时间：2025-06-25 作者：zyl 分享到：

文献：Research on mechanism of curcumin carried with PLGA-DSPE-PEG hybridized nanoparticles on regulating the nuclear receptor coactivator5 presentation, oxidative stress and presentation of alfa-fetoprotein in rats with liver cancer

文献链接：https://xueshu.baidu.com/usercenter/paper/show?paperid=1h0p0pr0ka6d0ma018430je0u1436527&site=xueshu_se

作者：作者：F Dai，W Chen，H Li

摘要：

This study intends to discuss the mechanism of curcumin carried with poly (lactic-co-glycolic acid)-1, 2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino (polyethylene glycol)-2000] (PLGA-DSPE-PEG) hybridized nanoparticles on regulating the Nuclear receptor coactivator5 (NCOA5) expression, oxidative stress and level of Alfa-fetoprotein (AFP) in rats with liver cancer. Curcumin carried with PLGA-DSPE-PEG hybridized nanoparticles was prepared. These rats were divided into normal set, model set, curcumin set and set of curcumin and nanoparticles randomly. The presentation of alanine transaminase (ALT) and aspartate aminotransferase (AST) in every set was detected. The pathological change in liver tissue with hematoxylin and eosin (H&E) staining method, level of malondialdehyde (MDA) and superoxide dismutase (SOD) was detected. Presentation of AFP and NCOA5 was detected with Western Blotting assay and real-time reverse transcription-polymerase chain reaction (RT-PCR).

本研究旨在探讨聚（丙交酯-共-乙醇酸）-1，2-二硬脂酰基-sn-甘油-3-磷酸乙酰胺-N-[氨基（聚乙二醇）-2000]（PLGA-DSPE-PEG）杂化纳米颗粒携带的姜黄素对*症大鼠核受体辅激活因子5（NCOA5）表达、氧化应激和α-铁蛋白（AFP）水平的调节作用机制。

制备了携带PLGA-DSPE-PEG杂化纳米颗粒的姜黄素。这些大鼠被随机分为正常组、模型组、姜黄素组和姜黄素和纳米粒组。检测每组中丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）的表现。

采用苏木精伊红（H&E）染色法检测肝组织病理变化，丙二醛（MDA）和超氧化物歧化酶（SOD）水平。采用Western Blotting法和实时逆转录聚合酶链式反应（RT-PCR）检测AFP和NCOA5的表达。正常组ALT、AST、MDA和AFP水平最高，姜黄素和纳米粒组最低。姜黄素和纳米颗粒组中的SOD呈现率最高。在正常设置下，表面纹理清晰，光泽度好，沿边缘排列有序。PLGA-DSPE-PEG杂交纳米粒子携带的姜黄素可降低*症大鼠的ALT和AST水平。SOD活性升高，MDA降低。AFP表达减少，NCOA5表达增加。