文献：Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes

作者：Nao Hiramoto-Yamaki, Kenji A. K. Tanaka, Kenichi G. N. Suzuki, Koichiro M. Hirosawa, Manami S. H. Miyahara, Ziya Kalay, Koichiro Tanaka, Rinshi S. Kasai … See all authors 

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Effects on DeffMACRO of Cy3-DOPE in the PtK2 PM

The DeffMACRO values of Cy3-DOPE were significantly increased and decreased by 30%, after the cells were treated with 5 µm latrunculin-B and 25 µm jasplakinolide, respectively (Figure 4, bottom right; Table 1), consistent with our previous observations in other cell types 19, 45, 48. These drug concentrations were much higher than those used previously, but PtK2 cells were extremely resistant to these drugs, and did not exhibit any morphological changes even under these conditions. Considering these results, along with the result showing a 20-fold increase of the Cy3-DOPE DeffMACRO value upon blebbing + actin depletion (Table 1), we concluded that the actin filaments associated with the PM cytoplasmic surface are responsible for slowing the diffusion of Cy3-DOPE in the PtK2 PM, as found previously in other cell types

PtK2粉末冶金中Cy3-DOPE对DeffMACRO的影响

在用5μmol/L的浓度处理细胞后，Cy3-DOPE的DeffMACRO值显著增加和减少了30% µm latrunculin-B和25 μm jasplakinolide（图4，右下；表1），与我们之前在其他细胞类型19、45、48中的观察结果一致。这些药物浓度远高于以前使用的药物浓度，但PtK2细胞对这些药物具有极强的耐药性，即使在这些条件下也没有表现出任何形态变化。

考虑到这些结果，以及起泡后Cy3 DOPE DeffMACRO值增加20倍的结果 + 肌动蛋白耗竭，我们得出结论，与PM细胞质表面相关的肌动蛋白丝负责减缓PtK2 PM中Cy3-DOPE的扩散，正如之前在其他细胞类型中发现的那样。

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