文献：Biotin-conjugated PEGylated porphyrin self-assembled nanoparticles co-targeting mitochondria and lysosomes for advanced chemo-photodynamic combination therapy†

作者：Baskaran Purushothaman , Jinhyeok Choi , Solji Park , Jeongmin Lee , Annie Agnes Suganya Samson , Sera Hong and Joon Myong Song

文献链接：https://pubs.rsc.org/en/content/articlehtml/2018/tb/c8tb01923a

摘要：

 The TPP–PEG–biotin chemical was synthesized by the acid–amine coupling reaction between biotin–PEG–COOH and TPP-amine. Briefly, 1 mmol of biotin–PEG–COOH, DMAP, EDCI and 3 ml of CH2Cl2 were added to a glass vial and the reaction mixture stirred for 3 h at 0 °C. Then 1 mmol of TPP-NH2 was added and the reaction mixture was stirred continuously for 36 h at room temperature under a N2 atmosphere. The unconjugated TPP-NH2 and the EDCI adduct formed were removed by column chromatography using silica gel. Finally, the pure TPP–PEG–biotin chemical was dried completely using a lyophilizer and characterized by spectroscopy methods. MALDI-TOF MS: m/z 4169.3 [M]+.

TPP-PEG-生物素的合成

通过生物素-PEG-COOH与TPP胺之间的酸胺偶联反应合成了TPP-PEG-生物素化学物质。简而言之，将1 mmol生物素-PEG-COOH、DMAP、EDCI和3 ml CH2Cl2加入玻璃瓶中，反应混合物在0°C下搅拌3小时。然后加入1mmol TPP-NH2，在室温下在N2气氛下连续搅拌反应混合物36小时。通过硅胶柱色谱法去除未偶联的TPP-NH2和形成的EDCI加合物。最后，使用冻干机将纯TPP-PEG-生物素化学物质完全干燥，并通过光谱法进行表征。MALDI-TOF MS:m/z 4169.3[m]+。

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