TPP-PEG-生物素自组装纳米粒子的制备-UDP糖丨MOF丨金属有机框架丨聚集诱导发光丨荧光标记推荐西安齐岳生物

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TPP-PEG-生物素自组装纳米粒子的制备

发布时间：2025-07-15 作者：zyl 分享到：

文献：Biotin-conjugated PEGylated porphyrin self-assembled nanoparticles co-targeting mitochondria and lysosomes for advanced chemo-photodynamic combination therapy†

作者：Baskaran Purushothaman , Jinhyeok Choi , Solji Park , Jeongmin Lee , Annie Agnes Suganya Samson , Sera Hong and Joon Myong Song

文献链接：https://pubs.rsc.org/en/content/articlehtml/2018/tb/c8tb01923a

摘要：

The NPs were prepared according to a previously published procedure.33 Briefly, 50 mg of TPP–PEG–biotin was dissolved in 5 ml of chloroform, dried under a flow of nitrogen gas, and dried further under vacuum to get a film layer. For nanoparticle synthesis, the TPP–PEG–biotin film layer was hydrated with 900 μl of PBS (pH 7.4) and 100 μl of DMSO. Then the solution mixture was sonicated for 15 min, and uniform sized self-assembled TPP–PEG–biotin NPs were obtained slowly. The NPs were collected by centrifugation at 15[thin space (1/6-em)]000 rpm using a TJ-25 centrifuge (Beckman Coulter). The characterization of TPP–PEG–biotin SANs was done by TEM and SEM. The stock was diluted with PBS (pH 7.4) and used for in vitro experiments.

TPP-PEG-生物素自组装纳米粒子的制备

NP是根据之前公布的程序制备的。将50 mg TPP–PEG–生物素溶解在5 ml氯仿中，在氮气流下干燥，然后在真空下进一步干燥以获得膜层。

对于纳米粒子合成，TPP-PEG-生物素膜层用900μl PBS（pH 7.4）和100μl DMSO水合。然后将溶液混合物超声处理15分钟，缓慢获得均匀大小的自组装TPP-PEG-生物素NP。

使用TJ-25离心机（Beckman Coulter）以15[薄空间（1/6-em）]000rpm的速度离心收集NP。通过透射电子显微镜（TEM）和扫描电子显微镜（SEM）对TPP-PEG-生物素-SAN进行了表征。将原液用PBS（pH 7.4）稀释，用于体外实验。