文献：Salivary Cortisol Determination on Smartphone-Based Differential Pulse Voltammetry System

作者：by Jingjing Liu 1,2,3ORCID,Ning Xu 2,Hong Men 2ORCID,Shuang Li 1ORCID,Yanli Lu 1,Sze Shin Low 1,Xin Li 1,Lihang Zhu 1,Chen Cheng 1,Gang Xu 1 andQingjun Liu

文献链接：https://www.mdpi.com/1424-8220/20/5/1422

摘要：

With the accelerated pace of life, non-invasive cortisol detection at the point of care (POC) is in high demand for personal health monitoring. In this paper, an ultrasensitive immunosensor using gold nanoparticles/molybdenum disulfide/gold nanoparticles (AuNPs/MoS2/AuNPs) as transducer was explored for non-invasive salivary cortisol monitoring at POC with the miniaturized differential pulse voltammetry (DPV) system based on a smartphone. Covalent binding of cortisol antibody (CORT-Ab) onto the AuNPs/MoS2/AuNPs transducer was achieved through the self-assembled monolayer of specially designed polyethylene glycol (PEG, SH-PEG-COOH). Non-specific binding was avoided by passivating the surface with ethanolamine. The miniaturized portable DPV system was utilized for human salivary cortisol detection. A series current response of different cortisol concentrations decreased and exhibited a linear range of 0.5–200 nM, the detection limit of 0.11 nM, and high sensitivity of 30 μA M−1 with a regression coefficient of 0.9947. Cortisol was also distinguished successfully from the other substances in saliva. The recovery ratio of spiked human salivary cortisol and the variation of salivary cortisol level during one day indicated the practicability of the immunosensor based on the portable system. 

本文探索了一种使用金纳米粒子/二硫化钼/金纳米粒子（AuNPs/MoS2/AuNPs）作为传感器的超灵敏免疫传感器，用于基于智能手机的小型差分脉冲伏安法（DPV）系统在POC进行非侵入性唾液皮质醇监测。

皮质醇抗体（CORT-Ab）与AuNPs/MoS2/AuNPs传感器的共价结合是通过特殊设计的聚乙二醇（PEG，SH-PEG-COOH）的自组装单层实现的。通过用乙醇胺钝化表面来避免非特异性结合。小型便携式DPV系统用于人类唾液皮质醇检测。

不同皮质醇浓度的一系列电流响应降低，线性范围为0.5-200 nM，检测限为0.11 nM，灵敏度为30μA M−1，回归系数为0.9947。皮质醇也成功地与唾液中的其他物质区分开来。加标人唾液皮质醇的回收率和唾液皮质醇水平在一天内的变化表明了基于便携式系统的免疫传感器的实用性。

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SH-PEG-HZ

ACA-PEG-SH

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MA-PEG-SH

N3-PEG-SH

SH-PEG-Biotin

Fmoc-NH-PEG-SH

SH-PEG-NH2.HCl

SH-PEG-COOH

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