文献：聚乙二醇-聚乙烯亚胺负载超顺磁纳米Fe3O4的合成及其基因转染应用

作者：姚琴，谢柏臻，裴一花

文献链接：https://xueshu.baidu.com/usercenter/paper/show?paperid=1y4b0j10670x08c077780mb0d3725607&site=xueshu_se

摘要：

A water-soluble graft copolymer polyethylene glycol grafted branched polyethylene imine (mPEG-g-PEI) was obtained by modifying polyethylene imine (PEI) with monomethyl ether polyethylene glycol (mPEG-OH), and polyethylene glycol polyethylene imine loaded with superparamagnetic iron oxide (PEG-PEI-SPIO) was prepared by ion exchange method. Then, α - carboxyl - ω - maleimide polyethylene glycol (mal-PEG-COOH) and multifunctional SPIO loaded antibody polyethylene glycol polyethylene imine (Ab PEG PEI-SPIO) were synthesized. The composite of PEG-PEI-SPIO and plasmid DNA (pDNA) had a particle size of about 105 nm. In vitro experiments showed that T cells could specifically absorb... The Ab-PEG-PEI loaded SPIO targeting group showed good performance in gene transfection experiments, and real-time non-invasive observation can be performed using magnetic resonance imaging during the gene transfection process

采用单甲基醚聚乙二醇(mPEG-OH)改性聚乙烯亚胺(PEI),得到水溶性接枝共聚物聚乙二醇接枝支化聚乙烯亚胺(mPEG-g-PEI),并利用离子交换法制备了负载超顺磁性氧化铁的聚乙二醇-聚乙烯亚胺(PEG-PEI-SPIO).然后合成了α-羧基-ω-马来酰亚胺聚乙二醇(mal-PEG-COOH)和多功能负载SPIO的抗体-聚乙二醇-聚乙烯亚胺(Ab-PEG-PEI-SPIO).PEG-PEI-SPIO与质粒DNA(pDNA)的复合物(Ab-PEG-PEI-SPIO/pDNA)粒径约105 nm.体外实验结果显示:T细胞能特异性地吸收Ab-PEG-PEI负载的SPIO,靶向组在基因转染实验中有较好的效果,在基因转染的过程中可用磁共振手段进行实时无创观测.

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