文献：Development of PLGA-PEG-COOH and Gelatin-Based Microparticles Dual Delivery System and E-Beam Sterilization Effects for Controlled Release of BMP-2 and IGF-1

作者：Yan Bai, Seyedsina Moeinzadeh, Sungwoo Kim, Youngbum Park, Elaine Lui, Hua Tan, Weiling Zhao, Xiaobo Zhou, Yunzhi Peter Yang

文献链接：https://onlinelibrary.wiley.com/doi/abs/10.1002/ppsc.202000180

摘要：

A poly(lactic-co-glycolic acid)-poly(ethylene glycol)-carboxyl (PLGA-PEG-COOH) and gelatin-based microparticles (MPs) dual delivery system for release of bone morphogenetic protein-2 (BMP-2) and insulin-like growth factor-1 (IGF-1) is developed. The delivery system is characterized based on its morphology, loading capacity, encapsulation efficiency, and release kinetics. The effects of electron beam (EB) sterilization on BMP-2 and IGF-1-loaded MPs and their biological effects are examined. The synergistic effect of a controlled dual release of BMP-2 and IGF-1 on osteogenesis of human mesenchymal stem cells (MSCs) is evaluated. Encapsulation efficiency of growth factors into gelatin and PLGA-PEG-COOH MPs is in the range of 64.78% to 76.11%. E-beam sterilized growth factor delivery systems are effective in significantly promoting osteogenesis of MSCs, although E-beam sterilization decreases the bioactivity of growth factors in MPs by ≈22%. BMP-2 release behavior from gelatin MPs/PEG hydrogel shows a faster release (52.7%) than that of IGF-1 from the PLGA-PEG-COOH MPs/PEG hydrogel (27.3%). The results demonstrate that the gelatin and PLGA-PEG-COOH MPs-based delivery system can realize temporal release of therapeutic biomolecules by incorporating different growth factors into distinct microparticles. EB sterilization is an accessible method for sterilizing growth factors-loaded carriers, which can pave the way for implementing growth factor delivery in clinical applications.

开发了一种用于释放骨形态发生蛋白-2（BMP-2）和胰岛素样生长因子-1（IGF-1）的聚乳酸-聚乙二醇-羧基（PLGA-PEG-COOH）和明胶基微粒（MP）双递送系统。

递送系统的特征在于其形态、负载能力、包封效率和释放动力学。研究了电子束（EB）灭菌对负载BMP-2和IGF-1的MP的影响及其生物学效应。评估了BMP-2和IGF-1的受控双重释放对人间充质干细胞（MSCs）成骨的协同作用。

生长因子在明胶和PLGA-PEG-COOH MP中的包封效率在64.78%至76.11%之间。电子束灭菌的生长因子递送系统能有效显著促进MSCs的成骨，尽管电子束灭菌会使MP中生长因子的生物活性降低约22%。

明胶MPs/PEG水凝胶的BMP-2释放行为比PLGA-PEG-COOH MPs/PEG凝胶的IGF-1释放行为（27.3%）更快（52.7%）。结果表明，基于明胶和PLGA-PEG-COOH MP的递送系统可以通过将不同的生长因子掺入不同的微粒中来实现治疗性生物分子的暂时释放。

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IA-PEG-COOH

AC-PEG-COOH

AlKyne-PEG-COOH

COOH-PEG-EPO

COOH-PEG-MA

COOH-PEG-N3

CHO-PEG-COOH

Biotin-PEG-COOH

Fmoc-NH-PEG-COOH

Boc-NH-PEG-COOH

Mal-PEG-COOH

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