文献：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy

作者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie

文献链接：https://onlinelibrary.wiley.com/doi/full/10.1155/2017/2849801

摘要：

Proper particle diameter [25, 26] and positive surface charge of nanodrug play an important role in cellular uptake of drug and enhancement target-oriented effects [17]. The particle size of FA-PEG-PEI@SPION-CDDP-TFPI-2 complexes was determined using TEM (Figure 4(a)). TEM micrograph illustrated monodisperse spherical particles with an average diameter of 55.2 ± 8.4 nm, the sizes of which contribute to avoiding phagocytosis by macrophages, renal excretion, and aggregation in the bone marrow. There were several SPION particles at an average diameter of 8 nm arranged as a ring inside the complex. Negligible sedimentation was observed in the complex liquid with one-year observation, which suggested excellent stability of the system. The TEM EDS in Figure 4(b) verified the presence of Pt and Fe elements in FA-PEG-PEI@SPION-CDDP-TFPI-2 complexes, suggesting a potent combination between CDDP and SPION. The Fe content and CDDP loading amount were 5.35 mg/ml and 100 ug/ml, respectively, as counted according to corresponding standard curves by colorimetry.

纳米药物的适当粒径和正表面电荷在细胞摄取药物和增强靶向效应中起着重要作用。粒径FA-PEG-PEI@SPION-CDDP-TFPI-2使用TEM测定复合物。

TEM显微照片显示了平均直径为55.2±8.4微米的单分散球形颗粒 nm，其大小有助于避免巨噬细胞的吞噬作用、肾脏排泄和骨髓中的聚集。有几个平均直径为8微米的SPION颗粒 nm在复合物内排列成环。经过一年的观察，在复杂的液体中观察到可忽略的沉淀，这表明该系统具有优异的稳定性。

TEM EDS验证了Pt和Fe元素在FA-PEG-PEI@SPION-CDDP-TFPI-2复合物，表明CDDP和SPION之间存在有效的结合。铁含量和CDDP负载量为5.35 mg/ml和100 ug/ml，根据相应的标准曲线通过比色法计数。

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