文献：CdSe量子点与蛋白质的作用研究

链接：https://www.opticsjournal.net/Articles/OJb53cd575dcbccca0/FullText

作者： 杨冬芝 1孙世安 2陈启凡 1徐淑坤 1

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以油酸为稳定剂,石蜡为还原剂,采用有机相法合成了尺寸均匀的CdSe量子点,并通过巯基乙酸将合成的量子点转移至水相.考查了CdSe量子点与几种结构不同的蛋白质(酶)之间的作用规律.研究发现,经巯基乙酸修饰后的量子点与牛血清白蛋白和胰凝乳蛋白酶作用后,荧光强度明显增大.而铜/锌-超氧化物歧化酶对量子点的荧光有明显的淬灭作用,牛血红蛋白对量子点的影响是随着时间的增加荧光强度先增大后减小,体现出一般蛋白质使荧光增强和部分金属离子使荧光淬灭两者的协同效应.

Using oleic acid as a stabilizer and paraffin as a reducing agent, CdSe quantum dots with uniform size were synthesized via the organic phase method, and the synthesized quantum dots were transferred to the aqueous phase through thioglycolic acid. The interaction patterns between CdSe quantum dots and several proteins (enzymes) with different structures were investigated. The study found that after interacting with bovine serum albumin and chymotrypsin, the fluorescence intensity of quantum dots modified with thioglycolic acid significantly increased. However, copper/zinc-superoxide dismutase had a significant quenching effect on the fluorescence of quantum dots, and the effect of bovine hemoglobin on quantum dots was that the fluorescence intensity first increased and then decreased with time, reflecting the synergistic effect of general proteins enhancing fluorescence and some metal ions quenching fluorescence

西安齐岳生物提供相关产品：

CdSe量子点

InP量子点

ZnSe量子点

PbS量子点

碳量子点

水溶性量子点

量子点荧光微球，包埋CdSe/ZnS量子点的二氧化硅微球

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