ProteOrange Kit with a fluorescent dye allows protein quantification with higher sensitivity than that of spectrophotometry and other conventional methods used for protein quantification.

ProteOrange reagent, the key kit ingredient, exhibits almost no fluorescence in the free form. In a solution it forms complexes with protein molecules and exhibits increased fluorescence upon binding to proteins. The excitation maximum of ProteOrange in the complex with protein is at ~485 nm, the emission maximum — at ~590 nm. The kit can be used with any fluorometer that has a suitable excitation source and detection channel. In general, the working range of measured protein concentration is limited by the equipment sensitivity. The recommended range of concentrations that can be measured is 10 ng/mL to 10 µg/mL for cuvette fluorometers and 100 ng/mL to 10 µg/mL for plate fluorometers.

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