近红外荧光染料IRDye 650 NHS Ester

IR 650 NHS酯（IRDye® 650 NHS酯类似物）是一种水溶性，明亮，远红荧光染料，光谱上类似于用于流式细胞术，显微镜和其他应用的其他650染料。IR 650 共轭物非常适合 633 nm 或 647 nm 激光线。与其他荧光团相比，使用长波长染料（如Cy5®染料或Alexa Fluor® 647染料）的显着优势是生物标本在该光谱区域中的自发荧光较低。

NHS酯（或琥珀酰亚胺酯）是最常用的胺反应基团，用于标记蛋白质伯胺（Lys），胺修饰寡核苷酸和其他含胺分子。蛋白质可以用IR 650 NHS酯以高摩尔比标记，而不会显着的自熄灭，从而导致更亮的偶联物和更灵敏的检测。IR 650偶联物可用于检测低丰度靶标。

　　ReactivityPrimary amine

　　Abs/Em Maxima651/668 nm

　　Extinction coefficient230,000 cm-1M-1

　　SolubilityWater, DMSO, DMF

　　Spectrally similar dyesCy5, Alexa Fluor® 647, DyLight® 649,

　　Molecular weight1032.18

　　Storage conditions-20C

　　Shipping conditionsAmbient temperature

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