FA-PEG-PEI功能化纳米复合材料在靶向递送顺铂和TFPI-2中的应用研究-UDP糖丨MOF丨金属有机框架丨聚集诱导发光丨荧光标记推荐西安齐岳生物

文献：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy

作者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie

文献链接：https://onlinelibrary.wiley.com/doi/full/10.1155/2017/2849801

摘要：

Our previous study has revealed that TFPI-2 is expressed at a low level in NPC cells and tissues [16]. As proof of concept we use FA as targeting molecules, which conjugated to amino-terminated polyethylene glycol (NH2-PEG-COOH) and polyethylenimine (PEI) to perform cationic polymers (FA-PEG-PEI) via amidation. Aldehyde sodium alginate modified Fe3O4 magnetic particles coated cisplatin (SPION-CDDP) was constructed with the above cationic polymer to obtain composite carriers (FA-PEG-PEI@SPION-CDDP), which then adsorbed TFPI-2 pDNA via electrostatic interaction to obtain the FA-targeted, CDDP, and TFPI-2-coated nanocomposites (FA-PEG-PEI@SPION-CDDP-TFPI-2). In vitro assays indicated that the novel compounds possessed excellent load-carrying capacity and drug stability and showed negligible cytotoxicity and enhanced targetability to FR positive (FR+) NPC HNE-1 cells.

FA-PEG-PEI

研究表明，TFPI-2在鼻咽癌细胞和组织中的表达水平较低。作为概念验证，我们使用FA作为靶向分子，它与氨基封端的聚乙二醇（NH2-PEG-COOH）和聚乙烯亚胺（PEI）共轭，通过酰胺化形成阳离子聚合物（FA-PEG-PEI）。

用上述阳离子聚合物构建了醛-海藻酸钠改性的Fe3O4磁性粒子包覆顺铂（SPION-CDDP），以获得复合载体(FA-PEG-PEI@SPION-CDDP)，然后通过静电相互作用吸附TFPI-2 pDNA，获得FA靶向、CDDP和TFPI-2涂覆的纳米复合材料(FA-PEG-PEI@SPION-CDDP-TFPI-2).体外试验表明，新化合物具有优异的承载能力和药物稳定性，对FR阳性（FR+）NPC HNE-1细胞的细胞毒性可以忽略不计，靶向性增强。